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KMID : 1007520230320111595
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Food Science and Biotechnology
2023 Volume.32 No. 11 p.1595 ~ p.1609
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Bacillus subtilis SPB1 lipopeptide biosurfactant: antibacterial efficiency against the phytopathogenic bacteria Agrobacterium tumefaciens and compared production in submerged and solid state fermentation systems
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Mouna Bouassida
Ines Mnif
Ines Hammami
Mohamed-Ali Triki
Dhouha Ghribi
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Abstract
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Bacillus subtilis SPB1 derived biosurfactants (BioS) proved its bio-control activity against Agrobacterium tumefaciens using tomato plant. Almost 83% of disease symptoms triggered by Agrobacterium tumefaciens were reduced. Aiming potential application, we studied lipopeptide cost-effective production in both fermentations systems, namely the submerged fermentation (SmF) and the solid-state fermentation (SSF) as well as the use of Aleppo pine waste and confectionery effluent as cheap substrates. Optimization studies using Box?Behnken (BB) design followed by the analysis with response surface methodology were applied. When using an effluent/sea water ratio of 1, Aleppo pine waste of 14.08 g/L and an inoculum size of 0.2, a best production yield of 17.16?±?0.91 mg/g was obtained for the SmF. While for the SSF, the best production yield of 27.59?±?1.63 mg/g was achieved when the value of Aleppo pine waste, moisture, and inoculum size were, respectively, equal to 25 g, 75%, and 0.2. Hence, this work demonstrated the superiority of SSF over SmF.
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KEYWORD
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Biocontrol, Biosurfactants, Aleppo pine waste, Fermentation, Response surface methodology
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